New Cyclohexenols and Benzopyran Derivatives from Fungus Aspergillus fumigatus F15ZA56

A chemical study of the fungus Aspergillus fumigatus F15ZA56 resulted in the elucidation of eight previously undescribed cyclohexenols, aspergienynes R-Y (1, 5–11), and three new benzopyran derivatives (2–4), together with two known analogues (12–13). The structures were determined based on HRESIMS and extensive NMR data. The absolute configurations of the chiral carbons in the new compounds were ultimately confirmed by ECD analysis. Bioactivity assays showed that compounds 4, 12 and 13 had significant inhibitory activities against TCPTP, PTP1B, and MEG2 (IC50 12.20–62.19 nM). Notably, compound 12 exhibited notable selectivity towards PTP1B (IC50 12.20 nM) over the tested phosphatases, comparable to the reference inhibitor AC484 (9.12 nM).