A novel protein, nuclear LIM interactor (NLI), was isolated and found to specifically associate with a single LIM domain in all nuclear LIM proteins tested and is expressed early in neuronal development.
LIM domain-containing transcription factors, including the LIM-only rhombotins and LIM-homeodomain proteins, are crucial for cell fate determination of erythroid and neuronal lineages. The zinc-binding LIM domains mediate protein-protein interactions, and interactions between nuclear LIM proteins and transcription factors with restricted expression patterns have been demonstrated. We have isolated a novel protein, nuclear LIM interactor (NLI), that specifically associates with a single LIM domain in all nuclear LIM proteins tested. NLI is expressed in the nuclei of diverse neuronal cell types and is coexpressed with a target interactor islet-1 (Isl1) during the initial stages of motor neuron differentiation, suggesting the mutual involvement of these proteins in the differentiation process. The broad range of interactions between NLI and LIM-containing transcription factors suggests the utilization of a common mechanism to impart unique cell fate instructions.
Jurata et al. (Tue,) reported a other. Nuclear LIM interactor (NLI) was evaluated on Protein-protein interactions and expression patterns of NLI. A novel protein, nuclear LIM interactor (NLI), was isolated and found to specifically associate with a single LIM domain in all nuclear LIM proteins tested and is expressed early in neuronal development.