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July 15, 2026Microbial Cell FactoriesOpen Access

Discovery of a dimeric isopimarane derivative by reexploring a labdane-related diterpenoid biosynthetic gene cluster in Aspergillus nidulans

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Authors

SLShu-Yi LinCJCory B. JenkinsonQHQuoc-Dung Tran Huynh

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Overview

Randomized trial unveils new isopimarane derivatives in Aspergillus nidulans, indicating novel biosynthetic potential.

Key Points

  • This research aims to investigate the diterpene biosynthesis pathway in Aspergillus nidulans and to identify new metabolites.
  • Reexplored the diterpene biosynthetic pathway using a multi-cluster deletion strain to reduce metabolite background.
  • Applied a modified alcA promoter system to drive the expression of a transcription factor that activates the biosynthetic gene cluster.
  • Identified new metabolites using liquid chromatography-mass spectrometry and nuclear magnetic resonance techniques.
  • Identified dipleosmarane, a new dimeric isopimarane derivative, alongside six known isopimarane diterpenoids.
  • Gene deletion of the terpene cyclase (pbcA) and geranylgeranyl pyrophosphate synthase (pbcB) confirmed the origin of the metabolites from the biosynthetic gene cluster.
  • The study demonstrates that A. nidulans primarily synthesizes isopimarane scaffolds instead of ent-pimarane derivatives.

Cite This Study

Lin et al. (2026) studied this question.

synapsesocial.com/papers/6a57242188b21df875480a40https://doi.org/10.1186/s12934-026-03056-0
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