Key result
CRISPR/Cas and Argonaute biosensors offer highly sensitive pathogen detection via targeted nuclease activation.
Why the study?
Pathogens pose a threat to human security, creating an urgent need for highly sensitive and specific approaches to rapidly identify and quantify them to prevent public health security outbreaks.
CRISPR/Cas and Argonaute systems offer promising guided, programmable, and targeted approaches for developing highly sensitive and specific biosensors for rapid pathogen detection.
May advance point-of-care pathogen detection; leaves open clinical validation and workflow integration.
Over the past few decades, pathogens have posed a threat to human security, and rapid identification of pathogens should be one of the ideal methods to prevent major public health security outbreaks. Therefore, there is an urgent need for highly sensitive and specific approaches to identify and quantify pathogens. Clustered Regularly Interspaced Short Palindromic Repeats CRISPR/Cas systems and Argonaute (Ago) belong to the Microbial Defense Systems (MDS). The guided, programmable, and targeted activation of nucleases by both of them is leading the way to a new generation of pathogens detection. We compare these two nucleases in terms of similarities and differences. In addition, we discuss future challenges and prospects for the development of the CRISPR/Cas systems and Argonaute (Ago) biosensors, especially electrochemical biosensors. This review is expected to afford researchers entering this multidisciplinary field useful guidance and to provide inspiration for the development of more innovative electrochemical biosensors for pathogens detection.
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Yang et al. (2023) conducted a review in Pathogen detection. CRISPR/Cas and Argonaute-based biosensors was evaluated. CRISPR/Cas and Argonaute-based biosensors offer guided, programmable, and targeted activation of nucleases for a new generation of highly sensitive and specific pathogen detection.
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