Key result
An optimized apical resection procedure combined with a whole-heart-slice approach provides a stable system to study neonatal heart regeneration and cardiomyocyte proliferation in situ.
Why the study?
Apical resection-induced neonatal heart regeneration is controversial due to variation in operative details across different laboratories.
An optimized apical resection procedure and whole-heart-slice analysis method provides a stable and reliable model for studying neonatal heart regeneration in mice.
Refines preclinical tools for neonatal cardiac regeneration studies in mice; leaves open translation to human therapies.
The neonatal heart completely regenerates after apical resection (AR), providing a desirable research model to study the mechanism of cardiac regeneration and cardiomyocyte proliferation. However, AR-induced neonatal heart regenerative phenomenon is controversial due to the variation of operative details in different laboratories. Here, we provide an optimized AR operation procedure with stable regeneration and high survival rate by achieving heart exposure, normalizing myocardium cut-offs, and reducing operation duration. We also established a whole-heart-slice approach to estimate the myocardial regeneration after the AR operation, which ensures no false-negative/positive results. The combination of the optimized AR operation and the whole-heart-slice analysis provides a stable system to study neonatal heart regeneration and cardiomyocyte proliferation in situ.
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Li et al. (2020) studied Myocardial regeneration. Optimized apical resection (AR) operation procedure and whole-heart-slice approach was evaluated on Stable myocardial regeneration and survival rate. An optimized apical resection procedure combined with a whole-heart-slice approach provides a stable system to study neonatal heart regeneration and cardiomyocyte proliferation in situ.
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