Key result
Refined neonatal mouse cardiac apicoectomy protocol improves survival and minimizes bleeding versus conventional models.
Why the study?
Conventional neonatal mouse heart apical resection models lack precise parameters for cryoanesthesia duration, thoracotomy site, and ambient temperature.
Does a refined apical resection protocol improve survival and reproducibility in neonatal mouse models of cardiac regeneration?
Does a refined apical resection protocol improve survival and reproducibility in neonatal mouse models of cardiac regeneration?
A refined neonatal mouse cardiac apicoectomy protocol improves survival and reproducibility for studying cardiac tissue regeneration.
Answers are grounded in this paper and cite their sources.
Establishes optimized cryoanesthesia parameters for reliable neonatal mouse AR models; extends preclinical tools but leaves open clinical translation.
Neonatal mouse heart apical resection (AR) can induce cardiac tissue regeneration. However, conventional AR models lack precise parameters for cryoanesthesia duration, thoracotomy site, and ambient temperature. We varied cryoanesthesia time, ambient temperature, and thoracotomy location to quantify blood loss, and survival rate. After surgery, RT-qPCR, immunofluorescence, and Masson staining were used to track AR model. Iterative testing showed that 4 min 30 s of cryoanesthesia followed by a 10-min delay at 24-28°C maintained complete cardiac arrest, providing a stable operative window. A fifth-intercostal thoracotomy, performed by gently compressing the lactating pup's back and dilating the thorax with blunt forceps, minimized bleeding and markedly improved survival. The refined AR protocol reproducibly elicits structural injury, transient functional impairment, and subsequent regenerative repair in P1 neonatal mouse hearts.
No takes yet. Share an insight, caveat, or question.
Ren et al. (2026) studied Cardiac tissue regeneration. Refined apical resection protocol vs. Conventional apical resection models was evaluated on Blood loss and survival rate. A refined neonatal mouse cardiac apicoectomy protocol using 4.5 minutes of cryoanesthesia and a fifth-intercostal thoracotomy minimized bleeding and improved survival.
Synapse has enriched 10 related papers. Consider them for comparative context: