Key result
NT-proBNP concentrations remained stable after storage at -80 °C for over 1 year and after up to five freeze-thaw cycles, with no significant difference between original and repeat values (P>0.05).
Why the study?
Is NT-proBNP stable after long-term storage at -80 °C and multiple freeze-thaw cycles in samples from healthy and heart failure patients?
Is NT-proBNP stable after long-term storage at -80 °C and multiple freeze-thaw cycles in samples from healthy and heart failure patients?
p-value: p=>0.05
NT-proBNP remains stable and immunoreactive after storage at -80 °C for over a year and after up to five freeze-thaw cycles, supporting its use in retrospective analyses.
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Supports retrospective NT-proBNP analyses with archived samples; leaves open prospective validation before routine clinical use.
Nowatzke et al. (2003) studied Healthy and heart failure (n=25). Storage at -80 °C and freeze-thaw cycles vs. Baseline (fresh) measurement was evaluated on Difference between original and repeat NT-proBNP values (p=>0.05). NT-proBNP concentrations remained stable after storage at -80 °C for over 1 year and after up to five freeze-thaw cycles, with no significant difference between original and repeat values (P>0.05).
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