What question did this study set out to answer?

To evaluate the long-term stability and immunoreactivity of endogenous BNP and NT-proBNP in frozen EDTA plasma samples stored at -20 °C without protease inhibitors over a four-month period.

January 1, 2004Clinical Chemistry and Laboratory Medicine (CCLM)

Long-term stability of endogenous B-type natriuretic peptide (BNP) and amino terminal proBNP (NT-proBNP) in frozen plasma samples

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Why the study?

Does storage at -20 degrees C without protease inhibitors affect the stability of endogenous BNP and NT-proBNP in plasma samples?

Population

60 EDTA plasma samples with initial BNP values between 30 and 420 pg/ml

Comparison

Storage at -20 degrees C without addition of… vs Initial concentrations determined within four…

Design

Other

Follow-up

120 days

Authors

TMThomas MuellerGeneral Cardiology AGAlfons GegenhuberInnsbruck Medical University BDBenjamin DieplingerAGO Austria

Discussion

Member takes

Overview

BNP results from -20°C-stored plasma without inhibitors may be unreliable; leaves open optimal storage protocols for future BNP research.

Key Points

To evaluate the long-term stability and immunoreactivity of endogenous BNP and NT-proBNP in frozen EDTA plasma samples stored at -20 °C without protease inhibitors over a four-month period.
Tested 60 EDTA plasma samples with baseline BNP concentrations between 30 and 420 pg/ml, initially measured within four hours of collection.
Stored samples at -20 °C without protease inhibitors and reanalyzed them using AxSYM BNP and Elecsys NT-proBNP assays after 1, 30, 60, 90, and 120 days.
Mean recovery of BNP was less than 70% after 1 day and dropped below 50% after 2 to 4 months of storage at -20 °C, with 3-month recovery ranging from 0% to 71%.
Mean recovery of NT-proBNP remained above 90% across all time intervals up to 120 days of storage at -20 °C, with 3-month recovery ranging from 91% to 112%.

Structured PICO

Does storage at -20 degrees C without protease inhibitors affect the stability of endogenous BNP and NT-proBNP in plasma samples?

Population

60 EDTA plasma samples with initial BNP values between 30 and 420 pg/ml

Intervention

Storage at -20 degrees C without addition of protease inhibitors for 1, 30, 60, 90, and 120 days

Comparator

Initial concentrations determined within four hours after blood collection

Outcome

Mean recovery (residual immunoreactivity) of BNP and NT-proBNP expressed in percent of the initial valuesurrogate

NT-proBNP is stable in frozen plasma at -20°C for at least four months, whereas BNP degrades rapidly and its measurement may not be valid under these conditions.

Cite This Study

Mueller et al. (2004) studied this question.

synapsesocial.com/papers/6a71a4145671bbf00bc2ee22 https://doi.org/10.1515/cclm.2004.153

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January 1, 2004Clinical Chemistry and Laboratory Medicine (CCLM)

Long-term stability of endogenous B-type natriuretic peptide (BNP) and amino terminal proBNP (NT-proBNP) in frozen plasma samples

View Full Paper

Ask AI

Bookmark

Why the study?

Does storage at -20 degrees C without protease inhibitors affect the stability of endogenous BNP and NT-proBNP in plasma samples?

Population

60 EDTA plasma samples with initial BNP values between 30 and 420 pg/ml

Comparison

Storage at -20 degrees C without addition of… vs Initial concentrations determined within four…

Design

Other

Follow-up

120 days

Authors

TMThomas MuellerGeneral Cardiology AGAlfons GegenhuberInnsbruck Medical University BDBenjamin DieplingerAGO Austria

Discussion

Member takes

Overview

BNP results from -20°C-stored plasma without inhibitors may be unreliable; leaves open optimal storage protocols for future BNP research.

Key Points

To evaluate the long-term stability and immunoreactivity of endogenous BNP and NT-proBNP in frozen EDTA plasma samples stored at -20 °C without protease inhibitors over a four-month period.
Tested 60 EDTA plasma samples with baseline BNP concentrations between 30 and 420 pg/ml, initially measured within four hours of collection.
Stored samples at -20 °C without protease inhibitors and reanalyzed them using AxSYM BNP and Elecsys NT-proBNP assays after 1, 30, 60, 90, and 120 days.
Mean recovery of BNP was less than 70% after 1 day and dropped below 50% after 2 to 4 months of storage at -20 °C, with 3-month recovery ranging from 0% to 71%.
Mean recovery of NT-proBNP remained above 90% across all time intervals up to 120 days of storage at -20 °C, with 3-month recovery ranging from 91% to 112%.

Structured PICO

Does storage at -20 degrees C without protease inhibitors affect the stability of endogenous BNP and NT-proBNP in plasma samples?

Population

60 EDTA plasma samples with initial BNP values between 30 and 420 pg/ml

Intervention

Storage at -20 degrees C without addition of protease inhibitors for 1, 30, 60, 90, and 120 days

Comparator

Initial concentrations determined within four hours after blood collection

Outcome

Mean recovery (residual immunoreactivity) of BNP and NT-proBNP expressed in percent of the initial valuesurrogate

NT-proBNP is stable in frozen plasma at -20°C for at least four months, whereas BNP degrades rapidly and its measurement may not be valid under these conditions.

Cite This Study

Mueller et al. (2004) studied this question.

synapsesocial.com/papers/6a71a4145671bbf00bc2ee22 https://doi.org/10.1515/cclm.2004.153

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