Brain natriuretic peptide is stable in whole blood and can be measured using a simple rapid assay: implications for clinical practice
Why the study?
Is BNP stable in whole blood at room temperature and can it be reliably measured using a rapid direct assay compared to conventional methods?
Population
42 subjects comprising 28 patients with left ventricular systolic dysfunction ranging from mild to severe…
Comparison
Storage of whole blood at room temperature over… vs Storage of plasma at room temperature…
Design
Other, Blinded
Follow-up
3 days (storage duration)
Authors
Discussion
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Member takes
Overview
Supports BNP testing from room-temperature whole blood after three days; leaves open broader validation before changing lab protocols.
Structured PICO
Is BNP stable in whole blood at room temperature and can it be reliably measured using a rapid direct assay compared to conventional methods?
P
Population
42 subjects (24 men, 18 women) comprising 28 patients with left ventricular systolic dysfunction (LVSD) ranging from mild to severe and 14 healthy volunteers.
I
Intervention
Storage of whole blood at room temperature over three days and measurement using a rapid, simple, direct (unextracted) BNP assay.
C
Comparator
Storage of plasma at room temperature; measurement of NT-ANP; conventional BNP assay using plasma extraction.
O
Outcome
Stability of NT-ANP and BNP when stored as whole blood or plasma at room temperature over three days.surrogate
BNP is stable in whole blood for three days at room temperature and can be measured using a rapid direct assay, facilitating routine clinical use without special sample handling.
Cite This Study
Murdoch et al. (1997) studied this question.