Key result
Mosaic depletion of serum response factor in neonatal murine cardiomyocytes disrupted sarcomere expansion, mitochondrial biogenesis, and T-tubule formation, demonstrating its essential role in maturation.
Population
Murine cardiomyocytes (neonatal and adult) in a mouse model
Design
Preclinical
Authors
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SRF regulates neonatal cardiomyocyte maturation in mice; leaves open its role in human cardiac development and disease.
Serum response factor (SRF) is identified as a critical, stage-specific regulator of murine cardiomyocyte maturation, primarily orchestrated through sarcomere assembly.
Guo et al. (2018) studied Cardiomyocyte maturation. AAV-mediated CRISPR/Cas9 depletion of Srf vs. Control (SrfF/+) was evaluated on Cardiomyocyte maturation (T-tubule formation, sarcomere expansion, mitochondrial biogenesis). Mosaic depletion of serum response factor in neonatal murine cardiomyocytes disrupted sarcomere expansion, mitochondrial biogenesis, and T-tubule formation, demonstrating its essential role in maturation.
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