Key result
Treatment with reducing agents like GSH and PDTC decreased MMP expression and increased TIMP levels in transformed fibroblast cells, while inducing MMP and repressing TIMP in normal fibroblasts.
Why the study?
Do reducing agents alter MMP expression, TIMP levels, and cell proliferation in normal and transformed human heart fibroblasts?
Population
Interstitial human heart fibroblast (HHF) and polyoma virus transformed fibroblast cells
Comparison
Thiol-containing reduced or oxidized… vs Cultured in medium in the absence of the…
Design
Preclinical
Authors
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Redox modulation of MMP/TIMP may differ by fibroblast phenotype; hypothesis-generating for remodeling therapies, needs in vivo confirmation.
Do reducing agents alter MMP expression, TIMP levels, and cell proliferation in normal and transformed human heart fibroblasts?
Reducing agents differentially regulate MMP and TIMP gene transcription in normal versus transformed human heart fibroblasts, suggesting a role for antioxidants in modulating cardiac remodeling and preventing tumorigenesis.
Tyagi et al. (1996) studied Normal and transformed fibroblast cells. Thiol and non-thiol reducing agents (GSH, PDTC, NAC, ascorbic acid) vs. Cultured without reducing agents was evaluated on MMP expression and TIMP levels. Treatment with reducing agents like GSH and PDTC decreased MMP expression and increased TIMP levels in transformed fibroblast cells, while inducing MMP and repressing TIMP in normal fibroblasts.
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