This protocol describes a method for purifying exosomes using sucrose gradient centrifugation of crude exosome pellets prepared by differential ultracentrifugation.Exosomes isolated by differential ultracentrifugation alone contain both exosomes and aggregated protein and nucleic acid contaminants.Sucrose gradient centrifugation effectively separates exosomes and other extracellular vesicles from these contaminants.
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Alexander et al. (2016) studied this question.
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