Imaging mitochondria live or fixed muscle tissues

Muscle tissue is particularly reliant on mitochondria for normal function.Mitochondrial morphology is important for function and altered structure is exhibited in a large number of pathologies.Imaging of mitochondria can therefore provide important information about tissue health and disease presence and progression.Here we describe several approaches to mitochondrial imaging in skeletal and cardiac muscle using confocal and two-photon microscopy including; tissue staining with IraZolve-Mito and MitoTracker ® Red CMXRos; antibody probing for Cytochrome C; and endogenous uorescence detections from NAD\(P)H localised to mitochondria.