Key result
A new optical coherence tomography approach accurately measured flow-mediated dilation in mouse femoral arteries at 11.96% ± 0.98%, which blunted to 5.69% ± 0.4% after L-NAME administration.
Absolute Event Rate: 11.96% vs 5.69%
A new OCT-based approach allows precise in vivo measurement of flow-mediated dilation in the mouse femoral artery, facilitating preclinical assessment of vascular endothelial function.
Supports preclinical mouse endothelial studies; leaves open human translation and validation.
Flow‐mediated vasodilation (FMD) is used for assessment of vascular endothelial function in humans as a predictor of cardiovascular events. It has been challenging to carry it on preclinical murine models due to the diminutive size of the femoral artery. Here, we present a new approach to accurately measure the blood velocity and femoral artery diameters of mice by acquiring Doppler optical coherence tomography and optical coherence tomography angiography continuously within 1 single experimental scanning protocol. Using the 3‐dimensional imaging and new velocity algorithm, the measurement precision of diameter, blood flow, velocity and wall shear stress are improved to 0.91%, 11.0%, 10.7% and 14.0%, respectively. FMD of healthy mouse femoral artery measured by this method was 11.96% ± 0.98%, which was blunted to 5.69% ± 0.4% by intravenous administration of endothelial nitric oxide synthase inhibitor (L‐N G ‐Nitroarginine methyl ester), in agreement with that reported in the literature.
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Song et al. (2018) studied Healthy mice. Optical coherence tomography (OCT) and OCT angiography vs. L-NAME administration was evaluated on Flow-mediated vasodilation (FMD). A new optical coherence tomography approach accurately measured flow-mediated dilation in mouse femoral arteries at 11.96% ± 0.98%, which blunted to 5.69% ± 0.4% after L-NAME administration.
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