March 9, 1998Circulation Research
Experimental Autoimmune Myocarditis Produced by Adoptive Transfer of Splenocytes After Myocardial Infarction
Key result
Adoptive transfer of concanavalin A-activated splenocytes from post-myocardial infarction rats into normal syngeneic rats produced cardiac-specific cellular infiltration and myocardial necrosis.
Why the study?
Does adoptive transfer of splenocytes from post-MI rats cause autoimmune myocarditis in normal syngeneic rats?
Population
Normal syngeneic rats
Design
Preclinical
Follow-up
6 weeks
Authors
Discussion
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Implication
Supports T-cell autoimmunity in experimental post-MI injury; leaves open relevance to human HF progression.
Key Points
- This research aims to examine the mechanism of T cell-mediated autoimmune myocarditis following myocardial infarction.
- Adoptive transfer of splenocytes from post-infarct rats to syngeneic normal rats.
- Purified splenocytes activated with concanavalin A and injected in varying doses.
- Histopathological studies conducted on recipient rats after 6 weeks.
- Evidence of lymphocyte-mediated myocardial injury observed in recipient rats.
- Infiltrate and necrosis correlated with donor infarct size.
- No infiltrates found in other organs; systolic function was normal.
Structured PICO
Does adoptive transfer of splenocytes from post-MI rats cause autoimmune myocarditis in normal syngeneic rats?
P
Population
Normal syngeneic rats receiving adoptive transfer of concanavalin A-activated splenocytes from rats 6 weeks post-myocardial infarction, followed for 6 weeks.
I
Intervention
Injection of concanavalin A-activated splenocytes purified from syngeneic rats 6 weeks post-myocardial infarction
O
Outcome
Histopathological evidence of lymphocyte-mediated myocardial injury (infiltrate and necrosis)surrogate
This study provides direct in vivo evidence that myocardial infarction can trigger a T cell-mediated autoimmune response against cardiac antigens, potentially contributing to post-infarction heart failure progression.
Cite This Study
Maisel et al. (1998) studied Myocardial infarction and autoimmune myocarditis. Adoptive transfer of concanavalin A-activated splenocytes from postinfarct rats was evaluated on Histopathological evidence of lymphocyte-mediated myocardial injury (infiltrate and necrosis). Adoptive transfer of concanavalin A-activated splenocytes from post-myocardial infarction rats into normal syngeneic rats produced cardiac-specific cellular infiltration and myocardial necrosis.