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This study aims to understand the role of human ubiquitin ligase POSH in HIV-1 assembly and secretion.

The trans-Golgi network-associated human ubiquitin-protein ligase POSH is essential for HIV type 1 production

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This study aims to understand the role of human ubiquitin ligase POSH in HIV-1 assembly and secretion.
Used small inhibitory RNA to silence hPOSH in cells, studying effects on HIV-1 production and Gag localization.
Reintroduced native hPOSH and a RING finger mutant to evaluate their impact on virus release and cellular distribution of Gag.
hPOSH silencing ablates virus secretion and disrupts Gag localization at the plasma membrane.
Reintroduction of native hPOSH restores both virus release and Gag localization, while the RING finger mutant inhibits these processes.
RING finger mutant hPOSH leads to the accumulation of intracellular Gag and prevents normal virus release.

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Randomized trial reveals hPOSH's essential role in HIV-1 production, indicating a potential therapeutic target.

Alroy et al. (2005) studied this question.

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