Patients with dilated cardiomyopathy had significantly greater myocardial TACE mRNA expression than control subjects (TACE/GAPDH ratio 2.52 vs 0.03, P<0.05).
Case-Control (n=35)
Increased myocardial TACE expression is associated with elevated TNF-alpha expression and correlates with clinical severity (lower LVEF and higher LVESD) in patients with dilated cardiomyopathy.
Absolute Event Rate: 2.52% vs 0.03%
p-value: p=<0.05
BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) has been implicated in the pathogenesis of dilated cardiomyopathy (DCM). TNF-alpha-converting enzyme (TACE) has recently been purified and its complementary DNA cloned. The expression of TACE results in the production of a functional enzyme that has precursor TNF-alpha in the mature form. The aim of this study was to determine whether TACE is expressed with TNF-alpha in myocardium and whether levels of TACE and TNF-alpha are related to clinical severity of DCM. METHODS AND RESULTS: Endomyocardial tissues were obtained from 30 patients with DCM and 5 control subjects. TNF-alpha and TACE mRNA levels were measured by a novel real-time quantitative reverse transcriptase-polymerase chain reaction method. Expression of TNF-alpha and TACE proteins was determined by immunohistochemical analysis. TNF-alpha mRNA was expressed in DCM patients (TNF-alpha/GAPDH ratio 0.85+/-0.24) but not in control subjects. TACE mRNA expression was significantly greater in DCM patients than in control subjects (TACE/GAPDH ratio 2.52+/-0.59 vs 0.03+/-0.02, P/=45 mm) showed significantly greater expression of TACE (P=0.02) and TNF-alpha (P=0. 001) than did the low LVESD subgroup (/=40%). CONCLUSIONS: This study has shown that increased myocardial TACE expression is associated with elevated myocardial TNF-alpha expression in both mRNA and protein levels in clinically advanced DCM.
Satoh et al. (Tue,) conducted a case-control in Dilated Cardiomyopathy (n=35). Dilated Cardiomyopathy vs. Control subjects was evaluated on TACE mRNA expression (TACE/GAPDH ratio) (p=<0.05). Patients with dilated cardiomyopathy had significantly greater myocardial TACE mRNA expression than control subjects (TACE/GAPDH ratio 2.52 vs 0.03, P<0.05).