Key result
Cotransfection of noninfected cells with nonreplicatable RNA derivatives led to the emergence of recombinant viral genomes, proving a viral RdRp-independent mechanism of RNA recombination.
Population
Cells infected with a replicating noncytopathogenic virus or noninfected cells
Design
Preclinical
Authors
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RdRp-independent recombination occurs in vitro; leaves open its contribution to natural viral evolution and genetic diversity.
The study demonstrates a viral RdRp-independent mechanism of RNA recombination in vivo, which may contribute to the evolution of RNA viruses and generation of recombinant cellular RNAs.
Gallei et al. (2004) studied RNA recombination in pestiviruses. Transfection of synthetic, nonreplicatable, subgenomic transcripts was evaluated on Generation of recombinant viral genomes. Cotransfection of noninfected cells with nonreplicatable RNA derivatives led to the emergence of recombinant viral genomes, proving a viral RdRp-independent mechanism of RNA recombination.
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