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Expression of TPM1 kappa is significantly lower compared to TPM1 alpha in both mouse heart and skeletal muscle, though its expression is higher in the heart than in skeletal muscle.
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Mouse TPM1 κ differences may limit cardiac model translatability; leaves open isoform roles in human striated muscle.
We have investigated the expression of TPM1 α and TPM1 κ in mouse striated muscles. TPM1 α and TMP1 κ were amplified from the cDNA of mouse heart by using conventional RT-PCR. We have cloned the PCR amplified DNA and determined the nucleotide sequences. Deduced amino acid sequences show that there are three amino acid changes in mouse exon 2a when compared with the human TPM1 κ . However, the deduced amino acid sequences of human TPM1 α and mouse TPM1 α are identical. Conventional RT-PCR data as well as qRT-PCR data, calculating both absolute copy number and relative expression, revealed that the expression of TPM1 κ is significantly lower compared to TPM1 α in both mouse heart and skeletal muscle. It was also found that the expression level of TPM1 κ transcripts in mouse heart is higher than it is in skeletal muscle. To the best of our knowledge, this is the first report of the expression of TPM1 κ in mammalian skeletal muscle.
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Dube et al. (2014) studied this question. TPM1 kappa and TPM1 alpha expression analysis was evaluated on Expression levels of TPM1 kappa compared to TPM1 alpha. Expression of TPM1 kappa is significantly lower compared to TPM1 alpha in both mouse heart and skeletal muscle, though its expression is higher in the heart than in skeletal muscle.
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