February 1, 1972Endocrinology
Measurement of Renin Activity, Concentration and Substrate in Rat Plasma by Radioimmunoassay of Angiotensin I
Key result
Radioimmunoassay of angiotensin I provided sensitive and specific serial measurements of renin activity, concentration, and substrate in small rat plasma samples, correlating well with bioassay.
Population
Rat plasma (including nephrectomized male rats)
Comparison
Radioimmunoassay of angiotensin I generated… vs Bioassay
Design
Preclinical
Authors
Discussion
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Implication
Enables serial renin monitoring in small rat samples; leaves open human translation.
Structured PICO
P
Population
Rat plasma (including nephrectomized male rats)
I
Intervention
Radioimmunoassay of angiotensin I generated during incubation of plasma in vitro
C
Comparator
Bioassay
O
Outcome
Measurement of renin activity, concentration, and substratesurrogate
The development of a sensitive radioimmunoassay for angiotensin I enables serial measurement of renin activity, concentration, and substrate in small plasma samples from individual rats.
Limitations
- Values for PRA and PRC measured by radioimmunoassay were higher than those observed by bioassay due to greater generation of angiotensin I under the incubation conditions.
Cite This Study
Ménard et al. (1972) studied this question. Radioimmunoassay of angiotensin I vs. Bioassay was evaluated on Measurement of renin activity, concentration, and substrate. Radioimmunoassay of angiotensin I provided sensitive and specific serial measurements of renin activity, concentration, and substrate in small rat plasma samples, correlating well with bioassay.