Key result
Human nebulin fragments containing six or more repeat modules bound to muscle actin with high affinity, and a six-module fragment bound to myosin but not tropomyosin, troponin, or tubulin.
Population
Human skeletal muscle nebulin cDNA fragments expressed in Escherichia coli
Design
Preclinical
Authors
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Nebulin repeat modules bind F-actin independently of number; extends in vitro mapping of sarcomeric interactions but leaves open in vivo roles.
Nebulin is a giant protein with numerous actin-binding sites that likely functions as a multifunctional template protein regulating thin filament length and participating in muscle activities.
Jin et al. (1991) studied this question. Human skeletal muscle nebulin cDNA fragments was evaluated on Protein interaction and binding affinity to muscle actin, myosin, and other proteins. Human nebulin fragments containing six or more repeat modules bound to muscle actin with high affinity, and a six-module fragment bound to myosin but not tropomyosin, troponin, or tubulin.
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