Key result
Insulin and growth hormone modified pyruvate metabolism in perfused rat hearts, including a rapid 7-fold activation of pyruvate dehydrogenase and a 5-fold reduction in [NAD]/[NADH] ratios.
Why the study?
Do insulin and growth hormone modify the flux of tracer from labelled lactate in perfused rat hearts?
Population
Perfused rat heart preparation
Comparison
Insulin and growth hormone vs Control
Design
Preclinical
Follow-up
up to 60 min
Authors
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Hypothesis-generating for cardiac pyruvate handling; leaves open translation to lactate flux or human therapy.
Do insulin and growth hormone modify the flux of tracer from labelled lactate in perfused rat hearts?
Insulin and growth hormone significantly alter pyruvate metabolism and reduce the [NAD]/[NADH] ratio in perfused rat hearts, supporting the compartmentation of glycolytic enzymes.
Mowbray et al. (1973) studied Perfused rat heart preparation. Insulin and growth hormone vs. Control was evaluated on Flux of tracer from labelled lactate, pyruvate dehydrogenase activation, and [NAD]/[NADH] ratios. Insulin and growth hormone modified pyruvate metabolism in perfused rat hearts, including a rapid 7-fold activation of pyruvate dehydrogenase and a 5-fold reduction in [NAD]/[NADH] ratios.