Key result
NASH was associated with significantly higher levels of IL-6, TNF-α, and CRP compared to healthy volunteers, and specific genotypes (IL-10 -1082 AA, TNF-α -308 A) correlated with altered cytokine levels.
Case-Control (n=96)
In patients with NASH, there is an imbalance between pro- and anti-inflammatory cytokines, and specific polymorphisms in IL-10 and TNF-α genes correlate with their plasma levels.
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Cytokine polymorphisms associate with NASH inflammation; leaves open causal role or therapeutic targeting.
NASH consists in lipid accumulation in hepatocytes that trigger oxidative stress, secretion of proinflammatory cytokines leading to steatohepatitis (NASH). The study aimed to investigate the levels of proinflammatory (TNF‐ α and IL‐6) along with anti‐inflammatory cytokine IL‐10 in patients with NASH and to correlate the cytokines’ level with their polymorphism. Sixty‐six patients with NASH and 30 healthy volunteers were included in the study. The plasmatic level of IL‐6, IL‐10, and TNF‐ α were determined by ELISA. IL‐10 ‐1082 G/A, IL‐6 ‐174 G/C, and TNF‐ α ‐308 G/A polymorphisms were determined using the PCR‐RFLP technique. IL‐6, TNF‐ α , and CRP levels were significantly higher in patients with NASH. There was a positive correlation between proinflammatory cytokines and a negative correlation between IL‐10 and proinflammatory markers. The G allele and GG genotype of IL‐6 ‐174 G/C polymorphism were more frequently noticed in NASH patients. Regarding IL‐10 ‐1082 G/A polymorphism, the AA genotype was correlated with NASH and with a low plasmatic level of IL‐10. The A allele in position 308 of the TNF‐ α gene was associated with high level of cytokine. In conclusion, there was an imbalance between pro‐ and anti‐inflammatory cytokines in NASH patients. IL‐10 ‐1082 G/A and TNF‐ α ‐308 G/A genotypes were correlated with the plasmatic levels of cytokines.
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Bocșan et al. (2017) conducted a case-control in Nonalcoholic Steatohepatitis (NASH) (n=96). Nonalcoholic Steatohepatitis (NASH) vs. Healthy volunteers was evaluated on Plasmatic levels of IL-6, IL-10, and TNF-α and their correlation with gene polymorphisms. NASH was associated with significantly higher levels of IL-6, TNF-α, and CRP compared to healthy volunteers, and specific genotypes (IL-10 -1082 AA, TNF-α -308 A) correlated with altered cytokine levels.
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