Key result
The goat TNNC1 gene was cloned and found to be highly conserved, with selective expression in cardiac tissue and slow skeletal muscle, but not in fast skeletal muscle or other organs.
Extends troponin conservation data to goats; leaves open utility for comparative cardiac models.
Cardiac troponin (TNNC1) is the calcium-binding subunit of the myofibril thin filament that regulates excitation-contraction coupling in cardiac muscle. In the study, we cloned TNNC1 gene of goat for the first time (GenBank accession number: HQ640744) and analyzed its tissue expression. Results indicate that TNNC1 was a 161-amino acid polypeptide that had been highly conserved during evolution. Its nucleotide sequence was high and was similar in various animals ranging from 97.94 (cow) to 83.33% (African clawed frog), and the identity of the encoded amino acids varied from 100 (cow) to 92.55% (smelt). Fluorescence quantitative PCR analyses revealed that the TNNC1 gene was selectively expressed in the muscular tissues of goat, was expressed in cardiac tissue and slow skeletal muscle (soleus), but was not expressed in fast skeletal muscle (longissimus muscle, gluteus maximus) and brain, kidney, lung or liver of goat. The amino acid residue of Ca 2+ binding site II is identical in
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Haolin Chen (2012) studied this question. Cloning and expression analysis of TNNC1 gene was evaluated on TNNC1 gene sequence and tissue expression. The goat TNNC1 gene was cloned and found to be highly conserved, with selective expression in cardiac tissue and slow skeletal muscle, but not in fast skeletal muscle or other organs.
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