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July 26, 2026STAR ProtocolsOpen Access

Protocol for SEC-based isolation and characterization of human plasma-derived extracellular vesicles

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Authors

FBFanni Annamária BorosPAPhilipp ArnoldMRMartin Regensburger

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Overview

This protocol presents a method to isolate and characterize extracellular vesicles in plasma, indicating further applications in biomarker research and therapy.

Key Points

  • To develop a protocol for the enrichment and characterization of human plasma-derived extracellular vesicles using size-exclusion chromatography.
  • Utilized size-exclusion chromatography (SEC) for enrichment of plasma-derived extracellular vesicles.
  • Employed western blot, transmission electron microscopy, and nanoparticle tracking analysis for characterization.
  • Outlined quality assessment steps for isolated EV samples.
  • Successfully enriched human plasma-derived EVs using SEC.
  • Characterization techniques including western blot and microscopy confirmed the presence and quality of the EVs.
  • Detailed methodologies facilitate further biomarker and therapeutic research.

Cite This Study

Boros et al. (2026) studied this question.

synapsesocial.com/papers/6a65a3a3d3aea3239cd76baehttps://doi.org/10.1016/j.xpro.2026.104735
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