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September 19, 2025ProteomesOpen Access

Comparative Analysis of Plasma Extracellular Vesicle Isolation Methods for Purity Assessment and Biomarker Discovery

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Authors

ASAlexandra T. StarMHMelissa HewittABAmanPreet Badhwar

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Overview

Systematic evaluation of isolation techniques highlights size-exclusion chromatography's advantages for biomarker discovery and purity assessment.

Key Points

  • Size-exclusion chromatography demonstrated the highest purity scores and EV marker enrichment.
  • PRM mass spectrometry reliably quantified key EV markers and contaminants across isolation methods.
  • Precipitation-based methods were less effective for proteomics-driven biomarker discovery.
  • Establishing a targeted purity scoring system aids in standardizing EV isolation quality for research and manufacturing.

Cite This Study

Star et al. (2025) studied this question.

synapsesocial.com/papers/68d46fbd31b076d99fa6994dhttps://doi.org/10.3390/proteomes13030045
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