Aging Impairs Reverse Remodeling and Recovery of Ventricular Function after Isoproterenol-Induced Cardiomyopathy

Figures

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  Figure 1. Echocardiographic parameters at baseline (black outlined bars) and after 28-day exposure to saline or isoproterenol infusion (blue outlined bars) in young and elderly mice. LVDd: End-diastolic left ventricular diameter; LVDs: End-systolic left ventricular diameter; IVS: interventricular septum thickness; PW: posterior wall thickness; EF: ejection fraction; FS: fractional shortening. N = 13 in both groups of young mice, and N = 14 in both groups of elderly mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

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  Figure 2. Histological findings and mRNA expression analyses at 28 days after exposure to isoproterenol or saline infusion in young and elderly mice. (A) Cardiomyocyte cross-sectional area (CSA) in the four study groups. A representative microphotograph for each one of them is shown (magnification ×40). N = 7/5 in sham/ISO-treated young mice; N = 7/5 in sham/ISO-treated elderly mice. (B) Quantification of collagen deposition in picrosirius-stained sections in the four study groups. Representative microphotographs are shown (magnification ×20). N = 5/7 in sham/ISO-treated young mice; N = 7/5 in sham/ISO-treated elderly mice. (C) mRNA expression of collagen I and collagen III in the four study groups. N = 8/6 in sham/ISO-treated young mice; N = 8/6 in sham/ISO-treated elderly mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

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  Figure 3. Echocardiographic parameters at baseline (black outlined bars), after 28-day exposure to saline or isoproterenol infusion (HF-28d, blue outlined bars) and at 56 days after exposure + recovery period (REC-56d, red outlined bars) in young and elderly mice. LVDd: End-diastolic left ventricular diameter; LVDs: End-systolic left ventricular diameter; IVS: interventricular septum thickness; PW: posterior wall thickness; EF: ejection fraction; FS: fractional shortening). N = 6/7 in sham/ISO-treated young mice; N = 3/4 in sham/ISO-treated elderly mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

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  Figure 4. Histological findings and mRNA expression analyses at 56 days, after exposure to isoproterenol or saline infusion and recovery, in young and elderly mice. (A) Cardiomyocyte cross-sectional area (CSA) in the four study groups. A representative microphotograph for each one of them is shown (magnification ×40). N = 5/5 in sham/ISO-treated young mice; N = 3/4 in sham/ISO-treated elderly mice. (B) Quantification of collagen deposition in picrosirius-stained sections in the four study groups. Representative microphotographs are shown (magnification ×20). (C) mRNA expression of collagen I and collagen III in the four study groups. N = 6/7 in sham/ISO-treated young mice; N = 3/4 in sham/ISO-treated elderly mice. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.