Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
July 28, 2026DiagnosticsOpen Access

Comparative Evaluation of Quantitative Real-Time PCR and a Laboratory-Developed Chip-Based Real-Time Digital PCR for JAK2 V617F Allele Burden in Myeloproliferative Neoplasms

View Full Paper
Ask AI
Bookmark
Share

Authors

SPSunggyun ParkKKKyoung Bo KimJHJung‐Sook Ha

Discussion

Loading...

Member takes

Overview

Randomized trial compares dPCR and qPCR for JAK2 V617F quantification in MPNs, indicating potential for improved detection.

Key Points

  • This research aims to compare the performance of a laboratory-developed dPCR assay with a commercial qPCR assay for quantifying JAK2 V617F variant allele frequency in myeloproliferative neoplasms.
  • Analyzed 76 JAK2 V617F-positive clinical specimens using both qPCR and dPCR.
  • Assessed analytical performance by limits of blank, detection, precision, linearity, and method comparison.
  • Examined associations between variant allele frequency and hematologic parameters.
  • Limit of detection was 0.1167% for qPCR and 0.0846% for dPCR.
  • dPCR showed lower variability and better precision at low allele burdens compared to qPCR.
  • Variant allele frequency was significantly higher in polycythemia vera relative to essential thrombocythemia.

Cite This Study

Park et al. (2026) studied this question.

synapsesocial.com/papers/6a6851062845b684d16f033bhttps://doi.org/10.3390/diagnostics16152333
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Laboratory-developed Droplet Digital PCR Assay for Quantification of the JAK2V617F Mutation2024 · 4 citations
  2. 2Superior detection of low-allele burden Janus kinase 2 V617F mutation and monitoring clonal evolution in myeloproliferative neoplasms using chip-based digital PCR2024 · 5 citations
  3. 3Polycythemia vera: 2024 update on diagnosis, risk‐stratification, and management2023 · 212 citations
  4. 4Clinical Implications of Quantitative <i>JAK2</i> V617F Analysis using Droplet Digital PCR in Myeloproliferative Neoplasms2017 · 9 citations
  5. 5Somatic <i>CALR</i> Mutations in Myeloproliferative Neoplasms with Nonmutated <i>JAK2</i>2013 · 1,764 citations