Key result
T lymphocytes from patients with immune thrombocytopenia exhibited increased lipid raft aggregation compared to negative controls, which promoted TCR-mediated T cell activation.
Observational
Abnormal lipid raft aggregation in T lymphocytes contributes to the pathogenesis of immune thrombocytopenia and can be blocked by MβCD in vitro.
Answers are grounded in this paper and cite their sources.
May implicate lipid rafts in ITP; hypothesis-generating and should not yet change practice.
Aberrant T lymphocytes signaling is considered to play a crucial role in the abnormal immune state of primary immune thrombocytopenia (ITP). Lipid raft has been verified to engage in the T cell receptor (TCR)-mediated T lymphocytes signal transduction. Whether lipid raft-associated T cells signal transduction has impact on the pathogenesis of ITP is still unconfirmed. In this study, we aimed to reveal the abnormality in structure and function of lipid rafts (LRs) in CD4(+) and CD8(+) T lymphocytes of patients with ITP. Our results showed that there was an increased lipid raft aggregation in ITP patients, while this kind of increase would not be influenced by platelet counts or therapeutic regimes. Stimulation by anti-CD3/CD28 monoclonal antibodies promoted enhanced lipid raft clustering in T lymphocytes of ITP patients compared with negative controls. Methyl-β-cyclodextrin (MβCD) could block the abnormal lipid raft aggregation and disrupt the TCR-mediated T cells proliferation and cytokines secretion, including both proinflammatory cytokines and anti-inflammatory cytokines. The spontaneous activation of T lymphocytes from ITP patients might be due to the elevated co-localization of protein tyrosine phosphatase (PTP) CD45 and lipid rafts in patients' CD4(+) and CD8(+) T lymphocytes. These findings suggest that the autoactivation of T lymphocytes from ITP patients may lead to the abnormality in lipid raft structure and raft-anchored proteins, and the changes conversely promote the TCR-mediated T cells activation of ITP patients.
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Zhang et al. (2015) conducted an observational in Primary immune thrombocytopenia (ITP). Primary immune thrombocytopenia vs. Negative controls was evaluated on Lipid raft aggregation and structure in CD4(+) and CD8(+) T lymphocytes. T lymphocytes from patients with immune thrombocytopenia exhibited increased lipid raft aggregation compared to negative controls, which promoted TCR-mediated T cell activation.
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