Key result
Shengmai injection attenuated oxidative stress-induced damage in neonatal rat cardiomyocytes, potentially through activation of the AKT pathway and inhibition of the ERK1/2 pathway.
Why the study?
Shengmai injection has long been used to treat heart failure, but scientific evidence for its anti-oxidative stress potential is lacking.
Does Shengmai injection alleviate H2O2-induced oxidative stress in neonatal rat cardiomyocytes?
Does Shengmai injection alleviate H2O2-induced oxidative stress in neonatal rat cardiomyocytes?
Shengmai injection attenuates oxidative stress-induced damage in neonatal rat cardiomyocytes by activating the AKT pathway and inhibiting the ERK1/2 pathway.
Adds preclinical support for pathway modulation in cardiomyocyte injury; leaves open any clinical translation.
ETHNOPHARMACOLOGICAL RELEVANCE: Shengmai injection (SMI) is a classical traditional Chinese medicine (TCM) officially recorded in Pharmacopoeia of the People's Republic of China (version 2015) and has long been used to treat heart failure in China. However scientific evidence for the anti-oxidative stress potential of SMI used in traditional medicine is lacking. AIM OF STUDY: was determined using Fura-2/AM and Rhod 2-AM fluorescent probe in mitochondrial in the process of oxidative stress. Annexin V-FITC/PI double staining was applied to examine the apoptotic cells in cardiomyocytes with oxidative stress. To identify the apoptosis after oxidative stress myocardial cells with the application of Annexin V-FITC/PI double staining apoptosis detection kit. Quantitative polymerase chain reaction (RT-PCR) was applied to measure the expression of antioxidant enzymes: catalase (CAT), superoxide dismutase (SOD), glutathione reductase (GSR). Western blot was performed to observe the phosphorylation of AKT and ERK1/2. RESULTS: -treated cardiomyocytes. Pretreatment with LY294002, an AKT phosphorylation inhibitor, suppressed the protective role of SMI in cardiomyocytes, while pretreatment with PD98059, an ERK1/2 phosphorylation inhibitor, enhanced the effect of SMI. CONCLUSIONS: In conclusion, SMI may attenuate oxidative stress-induced damage in cardiomyocytes potentially through the AKT and ERK1/2 pathway and can function as a promising injectable traditional Chinese medicine to treat oxidative stress-induced injury.
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Zhu et al. (2019) studied Oxidative stress-induced damage in cardiomyocytes. Shengmai injection (SMI) vs. Control (H2O2-induced oxidative stress without SMI) was evaluated on Apoptosis, expression of antioxidant enzymes, and phosphorylation of AKT and ERK1/2. Shengmai injection attenuated oxidative stress-induced damage in neonatal rat cardiomyocytes, potentially through activation of the AKT pathway and inhibition of the ERK1/2 pathway.
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