Key result
ACh-induced dilation was significantly enhanced in RGRR rats on a high-salt diet compared to SS rats, while dilation to SNP was similar in both strains.
Why the study?
Does transferring the renin gene chromosomal region restore normal vascular reactivity in Dahl salt-sensitive rats on a high-salt diet?
Does transferring the renin gene chromosomal region restore normal vascular reactivity in Dahl salt-sensitive rats on a high-salt diet?
Transferring the chromosomal region containing the renin gene restores ACh-induced arteriolar dilation in salt-sensitive rats on a high-salt diet, but does not alter sensitivity to elevated PO2.
Angiotensin II suppression impairs vascular relaxation on high-salt diet; leaves open whether renin gene transfer restores reactivity in salt-sensitive models.
OBJECTIVES: Angiotensin II suppression leads to impaired vascular relaxation in normotensive animals on a high-salt diet. The goal of this study was to determine whether normal vascular reactivity could be restored by transferring the chromosomal region carrying the Dahl salt-resistant (R) renin gene into the Dahl salt-sensitive (SS) genetic background in a strain of renin congenic rats (RGRR). METHODS: Male RGRR and SS rats were fed low-salt (0.4%) and high-salt (4%) diets for 4 weeks. The responses of cremaster muscle arterioles to acetylcholine (ACh), sodium nitroprusside (SNP), and elevated PO2 were assessed using video microscopy. RESULTS: ACh-induced dilation was significantly enhanced in RGRR on a high-salt diet compared to SS rats, while dilation to the NO donor SNP was similar in both strains. A high-salt diet significantly enhanced arteriolar constriction in response to elevated PO2, in both SS and RGRR rats. CONCLUSIONS: These data suggest that transfer of the chromosomal region containing the renin gene is crucial in the recovery of ACh-induced dilation of arterioles in RGRR rats vs. SS rats, and that factors in the SS genetic background contribute to an enhanced sensitivity to elevated PO2, independent of genes on chromosome 13.
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Drenjančević et al. (2004) studied Impaired vascular relaxation. Transfer of the chromosomal region carrying the Dahl salt-resistant (R) renin gene (RGRR strain) vs. Dahl salt-sensitive (SS) rats was evaluated on Responses of cremaster muscle arterioles to acetylcholine (ACh), sodium nitroprusside (SNP), and elevated PO2. ACh-induced dilation was significantly enhanced in RGRR rats on a high-salt diet compared to SS rats, while dilation to SNP was similar in both strains.
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