Key result
An 125I-labeled F-actin blot overlay assay identified a 17 kDa integral membrane protein (ponticulin) and a 30 kDa cytoplasmic protein as major F-actin-binding proteins in Dictyostelium discoideum.
The development of an 125I-labeled F-actin blot overlay assay allows for the specific identification of F-actin-binding proteins like ponticulin.
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Identifies ponticulin in Dictyostelium; leaves open relevance to human cardiovascular cell motility.
Chia et al. (1991) studied this question. 125I-labeled F-actin blot overlay assay was evaluated on Identification of F-actin-binding proteins. An 125I-labeled F-actin blot overlay assay identified a 17 kDa integral membrane protein (ponticulin) and a 30 kDa cytoplasmic protein as major F-actin-binding proteins in Dictyostelium discoideum.
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