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July 1, 1999Cardiovascular ResearchOpen Access

Cultured neonatal rat cardiac myocytes and fibroblasts do not synthesize renin or angiotensinogen evidence for stretch-induced cardiomyocyte hypertrophy independent of angiotensin II

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Key result

Cyclic stretch increased myocyte protein synthesis by 20% but was not accompanied by angiotensin II release, indicating stretch-induced hypertrophy is independent of angiotensin II.

Population

Myocytes and fibroblasts isolated by enzymatic dissociation from hearts of 1-3-day-old Wistar rat strain pups

Comparison

Exposure to cyclic stretch for 24 h vs Basal conditions (no stretch)

Design

Preclinical

Follow-up

24 hours

Authors

CKCharlotte van Kesteren

Discussion

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Overview

Questions autocrine Ang II release in stretched neonatal cardiomyocytes; leaves open RAS-independent hypertrophy mechanisms in adult human hearts.

Structured PICO

P
Population
Cultured cardiac myocytes and fibroblasts isolated from 1-3-day-old Wistar rat pups.
I
Intervention
Exposure to cyclic stretch for 24 h
C
Comparator
Basal conditions (no stretch)
O
Outcome
Synthesis of renin-angiotensin system (RAS) components (prorenin, renin, angiotensinogen, Ang I, Ang II) and myocyte protein synthesissurrogate

Neonatal rat cardiomyocytes and fibroblasts do not synthesize renin or angiotensinogen, demonstrating that stretch-induced hypertrophy occurs independently of local Ang II synthesis.

Cite This Study

Charlotte van Kesteren (1999) studied Cardiomyocyte hypertrophy. Cyclic stretch vs. Basal conditions was evaluated on Synthesis of renin-angiotensin system components and myocyte protein synthesis. Cyclic stretch increased myocyte protein synthesis by 20% but was not accompanied by angiotensin II release, indicating stretch-induced hypertrophy is independent of angiotensin II.

synapsesocial.com/papers/6a6a85c645e10bb5bcf2bde7https://doi.org/10.1016/s0008-6363(99)00057-7
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Left Ventricular Stretch Stimulates Angiotensin II– Mediated Phosphatidylinositol Hydrolysis and Protein Kinase C ε Isoform Translocation in Adult Guinea Pig Hearts1997 · 91 citations
  2. 2Prorenin, Renin, Angiotensinogen, and Angiotensin-Converting Enzyme in Normal and Failing Human Hearts1997 · 167 citations
  3. 3Renin-Angiotensin System Components in the Interstitial Fluid of the Isolated Perfused Rat Heart1997 · 164 citations
  4. 4Renin is not synthesized by cardiac and extrarenal vascular tissues. A review of experimental evidence.1994 · 180 citations
  5. 5Autocrine/Paracrine Determinants of Strain-activated Brain Natriuretic Peptide Gene Expression in Cultured Cardiac Myocytes1998 · 93 citations