What is the clinical evidence from this study?

Study design: Other. Population: Cultured rat cardiocytes. Intervention: Human recombinant interleukin-1 beta (IL-1 beta). Primary outcome: Synthesis of NO2-/NO3- (NOx) and expression of NOS mRNA and protein.

What question did this study set out to answer?

The aim is to understand how interleukin-1 beta affects nitric oxide synthase expression in cardiocytes.

July 1, 1994Circulation

Induction of nitric oxide synthase gene by interleukin-1 beta in cultured rat cardiocytes.

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Key result

Interleukin-1 beta dose-dependently and time-dependently induces macrophage-type inducible nitric oxide synthase (iNOS) mRNA expression and subsequent NO production in cultured rat cardiocytes.

Population

Cultured neonatal and adult rat cardiocytes

Comparison

Human recombinant interleukin-1 beta at doses of… vs Untreated control cells and cells treated with…

Design

Preclinical

Follow-up

up to 48 hours

Authors

MTMotoyoshi TsujinoTokyo Metropolitan Tama Medical Center YHYoko HirataGifu University TIT ImaiOgaki Municipal Hospital

Discussion

Member takes

Implication

May link IL-1β to septic myocardial depression via cardiac NOS; leaves open whether iNOS inhibition improves clinical outcomes.

Key Points

The aim is to understand how interleukin-1 beta affects nitric oxide synthase expression in cardiocytes.
Examined effects of interleukin-1 beta on nitric oxide production and NOS mRNA/protein expression in cultured rat cardiocytes over time.
Used Northern blot and Western blot analyses to assess iNOS mRNA and protein levels.
Tested NOS inhibitors and potential modulators, observing their impact on nitric oxide production.
IL-1 beta significantly increased NOx production in a dose- and time-dependent manner, peaking at 48 hours.
IL-1 beta induced iNOS mRNA expression, peaking at 48 hours in cardiocytes, with localized expression observed.
The NOS inhibitor completely blocked IL-1 beta-induced NOx production, which was confirmed to involve de novo protein synthesis.

Structured PICO

Population

Cultured neonatal and adult rat cardiocytes used to study the effect of interleukin-1 beta on nitric oxide synthase expression.

Intervention

Human recombinant interleukin-1 beta (IL-1 beta) at doses of 0.1 to 10 ng/mL for 6 to 48 hours

Comparator

Untreated control cells and cells treated with various inhibitors (NG-mono-methyl-L-arginine, dexamethasone, cycloheximide, actinomycin D, W-7, calphostin C, nicardipine)

Outcome

Synthesis of NO2-/NO3- (NOx) and expression of NOS mRNA and proteinsurrogate

IL-1 beta induces iNOS mRNA expression and de novo protein synthesis in cardiac myocytes, leading to excessive local NO production that may contribute to impaired contractility in septic shock.

Cite This Study

Tsujino et al. (1994) studied Cultured rat cardiocytes. Human recombinant interleukin-1 beta (IL-1 beta) was evaluated on Synthesis of NO2-/NO3- (NOx) and expression of NOS mRNA and protein. Interleukin-1 beta dose-dependently and time-dependently induces macrophage-type inducible nitric oxide synthase (iNOS) mRNA expression and subsequent NO production in cultured rat cardiocytes.

synapsesocial.com/papers/6a6a85c845e10bb5bcf2bdee https://doi.org/10.1161/01.cir.90.1.375

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Induction of nitric oxide synthase gene by interleukin-1 beta in cultured rat cardiocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Induction of nitric oxide synthase gene by interleukin-1 beta in cultured rat cardiocytes.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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