August 5, 2003Circulation Research
Cardiac Memory Is Associated With Decreased Levels of the Transcriptional Factor CREB Modulated by Angiotensin II and Calcium
Key result
AV sequential pacing induced cardiac memory associated with significantly decreased nuclear CREB and reduced CRE binding activity in the Kv4.3 promoter.
Why the study?
Does AV sequential pacing induce cardiac memory through decreased nuclear CREB modulated by angiotensin II and calcium in dogs?
Population
Dogs (AV sequentially paced or sham-operated)
Comparison
AV sequential pacing for 2 hours or 3 weeks… vs Sham-operated controls, or AVP dogs treated with…
Design
Preclinical
Follow-up
Up to 3 weeks
Authors
Discussion
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Implication
May inform pacing-induced remodeling mechanisms; hypothesis-generating for CREB in human cardiac memory.
Structured PICO
Does AV sequential pacing induce cardiac memory through decreased nuclear CREB modulated by angiotensin II and calcium in dogs?
P
Population
Dogs subjected to AV sequential pacing for 2 hours or 3 weeks to evaluate short- and long-term cardiac memory.
I
Intervention
AV sequential pacing (AVP) for 2 hours (for short-term cardiac memory) or 3 weeks (for long-term cardiac memory), with or without saralasin or nifedipine
C
Comparator
Sham-operated controls, or AVP dogs treated with saralasin or nifedipine
O
Outcome
Cardiac memory induction (evaluated with ECG and VCG) and nuclear CREB levels/CRE binding activitysurrogate
Cardiac memory induction is associated with decreased nuclear CREB levels, which is angiotensin-modulated and calcium-dependent, explaining the downregulation of Kv4.3 mRNA and Ito in long-term cardiac memory.
Cite This Study
Patberg et al. (2003) studied Cardiac memory. AV sequential pacing vs. Sham operation was evaluated on Nuclear CREB levels and CRE binding activity. AV sequential pacing induced cardiac memory associated with significantly decreased nuclear CREB and reduced CRE binding activity in the Kv4.3 promoter.