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July 30, 2026The Journal of Immunology

Integrating in silico Humanization and DNA Delivery to engineer anti-Influenza Monoclonal Antibodies for improved in vivo expression 2267274

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Authors

MTMartina TomirottiEGEbony GaryEPElizabeth Parzych

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Overview

Randomized trial demonstrates improved in vivo expression of humanized monoclonal antibodies in mice, suggesting enhanced pandemic preparedness.

Key Points

  • The aim is to develop humanized monoclonal antibodies against highly pathogenic avian influenza to enhance their functional properties and reduce immune reactions.
  • Developed a computational pipeline integrating BioPhi for humanization of murine antibody 5C2.
  • Generated and optimized a panel of humanized constructs for in vivo expression.
  • Delivered constructs as DNA-encoded monoclonal antibodies via injection and electroporation to mice.
  • In vitro expression confirmed for all humanized constructs.
  • DMAb treated mice showed strong neutralization against multiple H5N1 clades, including the circulating clade 2.3.4.4b.
  • Sustained expression of DMAbs observed for several months post-delivery.

Cite This Study

Tomirotti et al. (2026) studied this question.

synapsesocial.com/papers/6a6af56d60e2b924d3ea1afahttps://doi.org/10.1093/jimmun/vkag141.1128
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Also Consider

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  1. 1Expression and characterization of recombinant antibodies against H7 subtype avian influenza virus and their diagnostic potential2024 · 1 citations
  2. 2Characterization of Human Single-Chain Antibodies Against Highly Pathogenic Avian Influenza H5N1 Viruses: Mimotope and Neutralizing Activity2010 · 7 citations
  3. 3Prophylactic and therapeutic efficacy of monoclonal antibodies against H5N1 influenza virus2026 · 1 citations
  4. 4Direct airway delivery of a humanized anti-H7N9 neutralizing antibody broadly protects against divergent H7 influenza viruses in the mouse model2025
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