Key result
In HeLa cell extracts, poliovirus translation and processing were most efficient at 34 degrees C, while oleic acid and cerulenin inhibited viral synthesis, suggesting a need for intact membranes.
Population
HeLa cell extracts (cell-free system) for poliovirus synthesis
Comparison
Temperature variations and lipophilic agents vs Different temperatures or absence of lipophilic…
Design
Preclinical
Authors
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Cell-free poliovirus data remain hypothesis-generating; leaves open whether membrane integrity can be therapeutically targeted in vivo.
In vitro poliovirus translation, proteolytic processing, and virus formation are highly temperature-dependent and require intact membranes.
Molla et al. (1993) studied Poliovirus infection (in vitro). Temperature variations and lipophilic agents (cerulenin, oleic acid) vs. Other temperatures or no agents was evaluated on Poliovirus polyprotein translation, processing, and virus formation. In HeLa cell extracts, poliovirus translation and processing were most efficient at 34 degrees C, while oleic acid and cerulenin inhibited viral synthesis, suggesting a need for intact membranes.
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