September 1, 1983American Journal of Clinical Pathology
The Role of Anticoagulation in the Measurement of Platelet Volumes
Key result
Blood collected in ACD/Na2EDTA provided the most stable mean platelet volume over 8 hours, and an inverse correlation was observed between platelet count and MPV (r = -0.72, P<0.001).
Why the study?
Does the type of anticoagulant affect the stability of mean platelet volume measurements in blood samples?
Population
Blood samples from 52 volunteers
Comparison
Seven different anticoagulants vs Comparison among the different anticoagulants
Design
Preclinical
Follow-up
up to 8 hours
Authors
Discussion
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Implication
ACD/Na2EDTA tubes may optimize MPV stability for delayed testing; leaves open standardization of collection methods in platelet research.
Key Points
- This research investigates how different anticoagulants affect the stability of mean platelet volume (MPV) over time.
- Blood was collected in seven different anticoagulants and stored at room temperature for eight hours.
- Platelet counts, MPV, and other blood metrics were measured at various time points for stability analysis.
- Electrolyte composition, pH, and other factors were assessed for their influence on MPV stability.
- MPV was most stable in blood collected with 15% ACD and ACD/Na2 EDTA, remaining stable for up to eight hours.
- Heparin showed 16% variation in platelet counts, while sodium citrate produced the most variation in other metrics.
- An inverse correlation (r = -0.72, P < 0.001) was found between platelet count and MPV, suggesting the circulating platelet mass is crucial for homeostasis.
Study Design
Type
Observational (n=52)
Structured PICO
Does the type of anticoagulant affect the stability of mean platelet volume measurements in blood samples?
P
Population
52 volunteers whose blood was collected to study the effect of seven different anticoagulants on platelet size stability over 8 hours at room temperature.
E
Exposure
Seven different anticoagulants (including 15% ACD, ACD/Na2EDTA, Na2EDTA, K3EDTA, 11.9% ACD, sodium citrate, heparin)
C
Comparator
Comparison among the different anticoagulants
O
Outcome
Stability of mean platelet volume (MPV) over up to eight hours at room temperaturesurrogate
Main Result
Effect estimate: r = -0.72
p-value: p=<0.001
ACD/Na2EDTA provides the best conditions for stable measurement of mean platelet volume and platelet counts for up to eight hours at room temperature.
Cite This Study
Thompson et al. (1983) reported an observational. Anticoagulants (7 different types) was evaluated on Mean platelet volume (MPV) stability and correlation with platelet count (r = -0.72, p=<0.001). Blood collected in ACD/Na2EDTA provided the most stable mean platelet volume over 8 hours, and an inverse correlation was observed between platelet count and MPV (r = -0.72, P<0.001).