Key result
Sequencing links 2022 PeV-A3 isolates to a highly divergent clade with distinct mutations.
Continuous molecular surveillance through whole genome sequencing identifies newly emerging strains and evolutionary shifts in circulating parechoviruses.
We report the complete genome sequences of four parechovirus-A3 (PeV-A3) isolates from Children's Mercy Kansas City (CMKC), United States of America (USA): PeV-A3-MO-12-CMKC/CSF/MO/USA/2012 (isolated in 2012 from cerebrospinal fluid), PeV-A3-8C-CMKC/CSF/MO/USA/2022/ (isolated in 2022 from cerebrospinal fluid), PeV-A3-9C-CMKC/CSF/MO/USA/2022 (isolated in 2022 from cerebrospinal fluid), and PeV-A3-11B-CMKC/Blood/MO/USA/2022 (isolated in 2022 from blood). Sequence analysis revealed multiple mutations throughout the genome of the PeV-A3 isolates in comparison to the prototypic PeV-A3 A308/99 reference sequence (AB084913). Several unique amino acid changes were observed in the PeV-A3 isolates from 2022 that were absent in the PeV-A3 isolate from 2012. Phylogenetic analysis comparison determined that the sequenced PeV-A3 isolates from 2022 cluster together as a separate clade.
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Dhar et al. (2025) studied Parechovirus-A3 infection (n=4). Whole genome sequencing vs. Prototypic PeV-A3 reference sequence was evaluated on Genome sequences and phylogenetic clustering. Whole genome sequencing revealed that the 2022 PeV-A3 isolates cluster as a highly divergent, separate clade with unique amino acid substitutions compared to a 2012 isolate.
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