Key result
Addition of regulatory proteins increased unloaded sliding speed and isometric force, while the FHC TnT mutant increased sliding speed but reduced isometric force in single thin filaments.
Population
Reconstituted regulated actin filaments from rabbit F-actin, native bovine cardiac tropomyosin, and either…
Comparison
Addition of regulatory proteins, variation of… vs Unregulated thin filaments or native troponin
Design
Preclinical
Authors
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Basic in vitro motility data on troponin-tropomyosin regulation; leaves open relevance to intact cardiac function or clinical translation.
Thin filament regulatory proteins exert significant allosteric effects on crossbridge interactions, and the FHC-associated TnT mutation alters both sliding speed and isometric force.
Homsher et al. (2000) studied this question. Regulatory proteins and calcium (including FHC TnT mutant) vs. Unregulated thin filaments or wild-type TnT was evaluated on Unloaded sliding speed and isometric force. Addition of regulatory proteins increased unloaded sliding speed and isometric force, while the FHC TnT mutant increased sliding speed but reduced isometric force in single thin filaments.
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