What is the clinical evidence from this study?

Intervention: 5-hydroxytryptamine (5-HT) vs. predrug value. Primary outcome: Positive inotropic effect (contractility).

What question did this study set out to answer?

The aim is to investigate the receptor mechanisms mediated by 5-HT in rat cardiac preparations, particularly focusing on the left atrium.

March 1, 1998British Journal of PharmacologyOpen Access

Receptor mechanisms involved in the 5‐HT‐induced inotropic action in the rat isolated atrium

View Full Paper

Ask AI

Bookmark

Key result

5-HT exerted a positive inotropic effect in electrically driven rat left atria (312+/-50% of predrug value), mediated by ketanserin-sensitive 5-HT2A receptors rather than 5-HT4 receptors.

Population

Rat cardiac preparations (papillary muscles and electrically driven left atria)

Comparison

5-hydroxytryptamine up to 10 microM, with or… vs Baseline (predrug values)

Design

Preclinical

Authors

SLStephanie LäerHeinrich Heine University Düsseldorf FRFreerk Ole RemmersEppendorf (Germany)HSHasso ScholzMax Planck Society

Discussion

Member takes

Implication

Caution against human extrapolation from rat atria; leaves open 5-HT inotropy for translational studies.

Key Points

The aim is to investigate the receptor mechanisms mediated by 5-HT in rat cardiac preparations, particularly focusing on the left atrium.
Studied the effects of 5-HT on rat cardiac preparations including isolated left atria and papillary muscles.
Analyzed mRNA expression of 5-HT receptors using reverse transcriptase polymerase chain reaction with specific primers.
Investigated the impact of various antagonists on the positive inotropic effect of 5-HT.
5-HT at 10 microM increased contractility in electrically driven left atria to 312% of predrug value (n=8).
Only 5-HT2A receptor antagonist ketanserin diminished the inotropic effect, indicating its major role in this response.
5-HT4 receptor did not influence the inotropic effect, as confirmed by the lack of change in contractility with selective antagonists.

Structured PICO

Population

In vitro study of rat cardiac preparations (papillary muscles and left atria) to assess the inotropic effects of 5-HT.

Intervention

5-hydroxytryptamine (5-HT) up to 10 microM, with or without 5-HT4 receptor antagonists (GR 125487, SB 203186) or 5-HT2 receptor antagonist (ketanserin)

Comparator

Baseline (predrug values)

Outcome

Contractility (positive inotropic effect)surrogate

The positive inotropic effect of 5-HT in rat left atria is mediated by 5-HT2A receptors rather than 5-HT4 receptors.

Cite This Study

Läer et al. (1998) studied this question. 5-hydroxytryptamine (5-HT) vs. predrug value was evaluated on Positive inotropic effect (contractility). 5-HT exerted a positive inotropic effect in electrically driven rat left atria (312+/-50% of predrug value), mediated by ketanserin-sensitive 5-HT2A receptors rather than 5-HT4 receptors.

synapsesocial.com/papers/6a6c3eba547974b2dbf487c8 https://doi.org/10.1038/sj.bjp.0701702

View Full Paper

Ask AI

Bookmark

Receptor mechanisms involved in the 5‐HT‐induced inotropic action in the rat isolated atrium

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Receptor mechanisms involved in the 5‐HT‐induced inotropic action in the rat isolated atrium

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider