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June 19, 2018Frontiers in MicrobiologyOpen Access

Stimulation of the Internal Ribosome Entry Site (IRES)-Dependent Translation of Enterovirus 71 by DDX3X RNA Helicase and Viral 2A and 3C Proteases

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Key result

DDX3X RNA helicase positively regulated Enterovirus 71 IRES-dependent translation, and viral 2A and 3C proteases further enhanced this translation via their protease activities.

Population

Human muscle rhabdomyosarcoma cells, HEK293T, Huh7, and HeLa cells used as in vitro models for Enterovirus…

Comparison

Lentivirus-mediated shRNA knockdown of DDX3X… vs Control shRNA, mock infection, and wild-type DDX3X

Design

Preclinical

Authors

YSYu-Siang SuATAi-Hsuan TsaiYHYueh-Feng Ho

Discussion

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Overview

DDX3X may facilitate EV71 replication in vitro; leaves open its viability as an antiviral target pending further studies.

Structured PICO

P
Population
In vitro study using human cell lines to investigate the molecular mechanisms of Enterovirus 71 IRES-dependent translation.
I
Intervention
Lentivirus-mediated shRNA knockdown of DDX3X, expression of DDX3X helicase mutants (DQAD, AAA), and EV71 viral proteases (2Apro, 3Cpro)
C
Comparator
Control shRNA (shCtrl), mock infection, and wild-type DDX3X
O
Outcome
EV71 IRES-dependent translation activity (measured by luciferase reporter assays), viral titers, viral RNA levels, and viral protein expressionsurrogate

Main Result

p-value: p=<0.001

DDX3X is a critical RNA helicase that positively regulates EV71 IRES-dependent translation, a process further enhanced by viral 2A and 3C proteases.

Cite This Study

Su et al. (2018) studied Enterovirus 71 (EV71) infection. DDX3X knockdown and viral proteases (2A and 3C) vs. Control shRNA / mock infection was evaluated on IRES-dependent translation (measured by luciferase activity) (p=<0.001). DDX3X RNA helicase positively regulated Enterovirus 71 IRES-dependent translation, and viral 2A and 3C proteases further enhanced this translation via their protease activities.

synapsesocial.com/papers/6a6c8be526a7f98052db429ahttps://doi.org/10.3389/fmicb.2018.01324
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Canonical Eukaryotic Initiation Factors Determine Initiation of Translation by Internal Ribosomal Entry1996 · 499 citations
  2. 2Identification of cellular proteins enhancing activities of internal ribosomal entry sites by competition with oligodeoxynucleotides2004 · 46 citations
  3. 3Functional Dissection of Eukaryotic Initiation Factor 4F: the 4A Subunit and the Central Domain of the 4G Subunit Are Sufficient To Mediate Internal Entry of 43S Preinitiation Complexes1996 · 348 citations
  4. 4Cleavage of Poly(A)-Binding Protein by Enterovirus Proteases Concurrent with Inhibition of Translation In Vitro1999 · 268 citations
  5. 5Structural and functional analysis of the ribosome landing pad of poliovirus type 2: in vivo translation studies1991 · 186 citations