Key result
Modified and degenerate rotavirus G9-specific primers successfully typed previously untypable strains, revealing ≤99.4% genetic similarity to recent global G9 rotaviruses.
Observational
Yes
Genetic variation at the primer binding site explains typing failures for G9 rotaviruses, which can be overcome using modified primers.
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Improved G9 typing aids strain surveillance; leaves open clinical utility pending prospective validation.
Martella et al. (2003) conducted an observational in Severe rotavirus-associated enteritis. Modified (aFT9m) and degenerate (aFT9d) rotavirus G9-specific primers was evaluated on G typing by reverse transcription-polymerase chain reaction and genetic similarity. Modified and degenerate rotavirus G9-specific primers successfully typed previously untypable strains, revealing ≤99.4% genetic similarity to recent global G9 rotaviruses.
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