Key result
KCNE1 stabilizes the KCNQ1 S4 segment in the resting state, slowing modification 13-fold, while KCNE3 stabilizes the S4 segment in the active state.
Population
Human KCNQ1 channel with A226C mutation in the S4 segment
Comparison
Co-expression with KCNE1 or KCNE3 auxiliary… vs KCNQ1 channel expressed alone
Design
Preclinical
Authors
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Clarifies KCNE-KCNQ1 gating in animal models; leaves open translation to human channelopathies or therapies.
Effect estimate: 13 times slower with KCNE1
KCNE1 stabilizes the S4 segment of the KCNQ1 channel in the resting state and slows transition to the active state, whereas KCNE3 stabilizes it in the active state.
Nakajo et al. (2007) studied this question. KCNE1 and KCNE3 vs. Absence of KCNE1/KCNE3 was evaluated on Modification rate of A226C mutant by MTS (13 times slower with KCNE1). KCNE1 stabilizes the KCNQ1 S4 segment in the resting state, slowing modification 13-fold, while KCNE3 stabilizes the S4 segment in the active state.
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