Key result
Local application of weak acids or bases via a microperfusion system successfully created stable intracellular pH gradients as large as 1 pH unit within single ventricular myocytes.
Population
Single ventricular myocytes
Comparison
Local external application of NHCl, CO, or… vs Control solution stream
Design
Preclinical
Authors
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Facilitates mechanistic pH studies in isolated myocytes; leaves open translation to intact hearts or patients.
A novel microperfusion method successfully generates stable intracellular pH gradients in single cardiac myocytes, providing a tool to study localized acidosis effects such as in regional myocardial ischemia.
Spitzer et al. (2000) studied Single ventricular myocytes. Local external application of NH4Cl, CO2, or sodium propionate via microperfusion vs. Control solution was evaluated on Generation of intracellular pH gradients. Local application of weak acids or bases via a microperfusion system successfully created stable intracellular pH gradients as large as 1 pH unit within single ventricular myocytes.
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