Key result
Imposing an outward Na+ gradient induced rapid cytosolic acidification (t 1/2 = 44 s), while an inward gradient produced alkalinization, consistent with a rapid Na+-H+ exchange mechanism.
Population
Cultured growth-oriented embryonic chick heart cells
Comparison
Imposition of outward or inward Na+ gradients… vs Baseline conditions
Design
Preclinical
Authors
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Adapts microfluorometry for continuous pHi monitoring in contracting embryonic chick myocytes; leaves open validation in adult mammalian models.
This preclinical study demonstrates the presence of a rapid Na+-H+ exchange mechanism in the cardiac cell membrane that regulates intracellular pH.
Piwnica‐Worms et al. (1983) studied this question. Na+ gradient manipulation and Amiloride was evaluated on Intracellular pH (pHi) changes. Imposing an outward Na+ gradient induced rapid cytosolic acidification (t 1/2 = 44 s), while an inward gradient produced alkalinization, consistent with a rapid Na+-H+ exchange mechanism.
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