Key result
Low buffered superfusion of isolated muscle causes surface acidosis, depressing intracellular pH and twitch tension.
Why the study?
The relationship between surface pH and intracellular pH regulation in cardiac and skeletal muscle under varying extracellular conditions was not fully characterized.
Does superfusion with low buffered solutions or weak acids/bases alter surface and intracellular pH in isolated cardiac and skeletal muscle?
Does superfusion with low buffered solutions or weak acids/bases alter surface and intracellular pH in isolated cardiac and skeletal muscle?
Surface acidification in cardiac and skeletal muscle preparations leads to intracellular acidification and depressed twitch tension, highlighting the role of surface pH in regulating intracellular pH and contractility.
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Surface pH shifts may confound isolated muscle contractility assays; leaves open in vivo or clinical relevance.
Hemptinne et al. (1987) studied this question. Superfusion with low buffered solution was evaluated on Surface pH (pHs) and intracellular pH (pHi). Superfusion of isolated cardiac and skeletal muscle with a low buffered solution caused surface acidosis, which induced a slower acid change of intracellular pH and depressed twitch tension.
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