Key result
The amino acid at position 57 of the leader protein (L57) regulates subgroup-specific Theiler's murine encephalomyelitis virus growth and viral RNA encapsidation on BHK-21 cells.
The amino acid at position 57 of the TMEV leader protein regulates subgroup-specific virus growth on BHK-21 cells, likely by affecting viral RNA encapsidation efficiency.
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L(57) regulates TMEV growth in BHK-21 cells; leaves open its role in subgroup-specific pathogenesis.
Strains of Theiler's murine encephalomyelitis virus (TMEV) are divided into two subgroups, TO and GDVII. TMEV strains show subgroup-specific virus growth and cell tropism and induce subgroup-specific diseases. Using site-directed mutagenesis, we demonstrated that the amino acid at position 57 of the leader protein (L(57)), which is located at the most N-terminal part of the polyprotein, regulates subgroup-specific virus growth on BHK-21 cells. Further study suggested that L(57) may regulate viral RNA encapsidation, although it does not affect the synthesis of viral proteins or the assembly of viral intermediates.
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Takano‐Maruyama et al. (2006) studied Theiler's murine encephalomyelitis virus (TMEV) infection. Amino acid substitution at position 57 of the leader protein (L57) vs. Wild-type virus strains (DA and GDVII) was evaluated on Virus growth on BHK-21 cells (viral titer and plaque size). The amino acid at position 57 of the leader protein (L57) regulates subgroup-specific Theiler's murine encephalomyelitis virus growth and viral RNA encapsidation on BHK-21 cells.
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