August 27, 2009Journal of VirologyOpen Access

Random Mutagenesis Defines a Domain of Theiler's Virus Leader Protein That Is Essential for Antagonism of Nucleocytoplasmic Trafficking and Cytokine Gene Expression

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Structured PICO

Population

In vitro and in vivo models of Theiler's murine encephalomyelitis virus (TMEV) infection

Intervention

Mutant forms of the TMEV leader protein generated by random mutagenesis (specifically targeting the Theilo domain)

Comparator

Wild-type TMEV leader protein

Outcome

Impact on TMEV L protein activity (interferon gene transcription, IRF-3 dimerization antagonism, nucleocytoplasmic trafficking, nucleoporin 98 hyperphosphorylation, and viral persistence in vivo)surrogate

Identification of the Theilo domain in the TMEV leader protein reveals its essential role in antagonizing host immune responses and establishing persistent infection.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Theilo domain mutations disrupt TMEV L protein functions; hypothesis-generating for viral persistence mechanisms, should not yet inform therapies.

Cite This Study

Ricour et al. (2009) studied this question.

synapsesocial.com/papers/6a854821895c7e29f4c6bb8e https://doi.org/10.1128/jvi.00829-09

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Implication

Theilo domain mutations disrupt TMEV L protein functions; hypothesis-generating for viral persistence mechanisms, should not yet inform therapies.

Random Mutagenesis Defines a Domain of Theiler's Virus Leader Protein That Is Essential for Antagonism of Nucleocytoplasmic Trafficking and Cytokine Gene Expression

Structured PICO

Discussion

Implication

Cite This Study

Random Mutagenesis Defines a Domain of Theiler's Virus Leader Protein That Is Essential for Antagonism of Nucleocytoplasmic Trafficking and Cytokine Gene Expression

Structured PICO

Discussion

Implication

Cite This Study

Also Consider

Also Consider