By cultivating the Pasteur Institute strain of Clostridium tetani in cellophane bags immersed in Weinberg’s Vf broth, toxins containing from 600,000 to 1,200,000 guinea pig minimum lethal doses per milliliter have been obtained. Without the cellophane, the same strain and the same medium gave not more than 100,000 guinea pig M.L.D. per milliliter.
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Fredette et al. (1952) studied this question.